Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Feature | Source |
|---|---|---|
| Xanthonomans citri ssp. citri | ||
| Xcc306WT | Xcc306 Group A, wild‐type, Rif | DPI* |
| Xcc306ΔpthA4 | pthA4 deletion mutant | Jeffery Johne's laboratory (Gainesville, FL, USA) |
| Xcc306ΔpthA4::dCsLOB1 |
Artificial TALE targeting CsLOB1 complement Xcc306ΔpthA4, Gm |
This study |
| Xcc306ΔpthA4::dCsLOB2 |
Artificial TALE targeting CsLOB2 complement Xcc306ΔpthA4, Gm |
This study |
| Xcc306ΔpthA4::dCsLOB2L |
Artificial TALE targeting CsLOB2 complement Xcc306ΔpthA4, Gm |
This study |
| Xcc306ΔpthA4::dCsLOB3 |
Artificial TALE targeting CsLOB3 complement Xcc306ΔpthA4, Gm |
This study |
| Xcc306ΔpthA4::dCsLOB4/Xcc306ΔpthA4::dCsLOB4‐2 |
Artificial TALE targeting CsLOB4 complement Xcc306ΔpthA4, Gm |
This study |
| Xanthomonas campestris pv. vesicatoria | ||
| Xcv8510 | Wild‐type | Jeffery Johne's laboratory (Gainesville, FL, USA) |
| Xcv8510::pHMI | Empty vector pHMI complement Xcv8510 | This study |
| Xcv8510:: dCsLOB2 |
Artificial TALE targeting CsLOB2 complement Xcv8510, Gm |
This study |
| Escherichia coli | ||
| DH5α | F−recAϕ80dlacZΔM15 | New England Biology (NEB) |
| Plasmid | ||
| pBluescript KS(+) | Phagemid, pUC derivative, Ampr | Stratagene |
| pHMI | Broad‐host‐range resistance to Sp | Hopkins et al. (1992) |
| pUFR053 | repW, Mob+, LacZα+, Par+, Gmr | Clontech |
Ampr, ampicillin‐resistant; Gm, gentamicin; Sp, spectinomycin; Rif, rifamycin.
*DPI, Division of Plant Industry of the Florida Department of Agriculture and Consumer Services (Gainesville, FL, USA).
†BRL, Bethesda Research Laboratories (Gaithersburg, MD, USA).