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. 2016 Jul 22;18(2):249–262. doi: 10.1111/mpp.12397

Figure 4.

Figure 4

YtrA regulates the expression of the bacterial type III secretion system (T3SS) and type III effectors (T3Es). (A) YtrA positively regulates T3SS in Xanthomonas citri ssp. citri (Xac) in planta. The wild‐type and mutant were inoculated in Duncan grapefruit. The relative expression (mutant vs. wild‐type) of the genes of interest at different time points is shown as relative values on a logarithmic scale. (B) Ectopic expression of hrpG and hrpX restored the deficient virulence in the ytrA mutant. Xac strains were inoculated in the host plant grapefruit with starting concentrations of OD600 = 0.3 or 0.03. Inoculated leaves were photographed at 3 days post‐inoculation (dpi). (C) The bacterial population in planta was tested at 6 dpi. Black and white columns indicate that strains were inoculated with starting concentrations of OD600 = 0.03 and 0.3 respectively. WT, Xac 306 with empty vector pUFR053; M, ytrA mutant with pUFR053; MC, ytrA mutant with p53‐YtrA; MC‐X, ytrA mutant with p53‐HrpX; MC‐G, ytrA mutant with p53‐HrpG.