Figure 1.

Stress response phenotypes of Fusarium oxysporum mitogen‐activated protein kinase (MAPK) mutants. (A, C) Colony phenotypes of the indicated strains grown on YPD (3% yeast extract, 1% peptone, 2% glucose and 1.5% agar) medium at different temperatures (28 or 34 ºC) or at 28 ºC in the presence of the indicated compounds. Plates were spot inoculated with the indicated number of microconidia, incubated for 2 days at 28 ºC or 4 days at 34 ºC, and scanned. Bar, 1 cm. (B, D) Quantitative analysis of the colony area (cm²) performed using MultiGauge software. Data were analysed and plotted with the program GraphPad Prism version 5. Error bars represent standard deviations of colony areas calculated from four independent replicates. Values with the same letter are not significantly different according to Dunnett's multiple comparison test (P ≤ 0.05). CFW, calcofluor white; CR, Congo red; wt, wild‐type.