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. 2016 Jul 27;18(5):649–661. doi: 10.1111/mpp.12425

Figure 7.

Figure 7

Transcriptional changes in the defence‐related genes TaPR1.1 (a), TaPIE1 (b) and TaAOS (c) in TaWRKY70‐induced [under high‐temperature (HT, 20 ºC) treatment] wheat leaves during the initial symptom expression stage of Puccinia striiformis f. sp. tritici development. The expression levels of these genes were determined by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). The presented transcript levels are relative to the reference gene 26S (ATP‐dependent 26S proteasome regulatory subunit). The bars show standard errors. Analysis of variance (ANOVA) was conducted by Duncan's multiple range test (P = 0.01) to analyse the gene expression differences among different vector‐inoculated plants for each time point; the means of the gene expression levels do not differ significantly if they are indicated by the same lowercase letter.