Figure 1.

Wounding promotes both the up‐regulation of jasmonic acid (JA) biosynthesis‐related gene expression and oxo‐phytodienoic acid (OPDA)/JA accumulation in cotton cotyledons. (A) Twelve‐day‐old cotton plants were wounded four times with a haemostat. Damaged cotyledons were harvested at the indicated time points after wounding. OPDA/JA levels were determined by liquid chromatography‐mass spectrometry (LC‐MS). (B) Expression of cotton ALLENE OXIDE SYNTHASE‐like (GhAOS‐like), ALLENE OXIDE CYCLASE 2‐like (GhAOC2‐like) and ACYL‐COENZYME A OXIDASE‐like 1a (GhACX1a‐like) genes in damaged cotyledons was quantified by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). Results in (A) and (B) are averages of three biological replicates; error bars indicate standard deviation (n > 12).