. 2016 Dec 18;18(9):1282–1294. doi: 10.1111/mpp.12492

Table 1.

Phenotypes of the parental strain zj‐1, deletion mutants ΔFaCdc3‐5 and ΔFaCdc12‐71, and complemented strains ΔFaCdc3‐5C and ΔFaCdc12‐71C of Fusarium asiaticum in terms of growth, conidiation, deoxynivalenol (DON) production, perithecia production and virulence.

Strain	Growth rate on two media (mm/day)*		Conidiation (× 10⁵ mL)	DON production (μg/g) ^†	Perithecium covered in each seed ^‡	Percentage of diseased spikelets ^§
Strain	PSA	CM	Conidiation (× 10⁵ mL)	DON production (μg/g) ^†	Perithecium covered in each seed ^‡	Percentage of diseased spikelets ^§
zj‐1	26.44 ± 0.2^a	23.4 ± 0.2^a	4.43 ± 0.58^a	2.24 ± 0.13^a	+++	23.14 ± 2.19^a
ΔFaCdc3‐5	9.3 ± 0.1^b	8.7 ± 0.1^b	0.12 ± 0.05^b	0.91 ± 0.04^b	+	5.37 ± 1.29^b
ΔFaCdc3‐5C	25.7 ± 0.4^a	23.2 ± 0.7^a	4 ± 0.36^a	2.13 ± 0.13^a	+++	25.83 ± 5.15^a
ΔFaCdc12‐71	8.3 ± 0.4c	7.2 ± 0.3c	0.15 ± 0.09^b	0.69 ± 0.07^b	+	4.42 ± 1.55^b
ΔFaCdc12‐71C	25.9 ± 0.7^a	22.8 ± 0.8^a	4.13 ± 0.40^a	2.09 ± 0.13^a	+++	23.97 ± 3.55^a

CM, complete medium; PSA, potato sucrose agar.

*Growth rate and conidiation were measured after incubation for 3 and 7 days, respectively. The mean and standard deviation were calculated from three replicates. The same letter indicates that there is no significant difference. Different letters are used to mark statistically significant differences (P = 0.05).

^†DON production in glucose–yeast extract–peptone (GYEP) inoculated with 1 × 10⁵ spores and cultured for 7 days. Data were collected from three replicates.

^‡Perithecium covered in each seed is scored as ‘+++’, ‘++’ or ‘+’ when the perithecium covered more than two‐thirds, between one‐third and two‐thirds, or less than one‐third of each seed.

^§Average percentage of diseased spikelets per spike at 15 days after inoculation. Thirty spikes were inoculated for each strain.