Figure 2.

Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) analysis of CaLRR51 in pepper plants during Ralstonia solanacearum infection and exogenous phytohormone application. For R. solanacearum infection assay, the third leaves from the top of pepper plants at the eight‐leaf stage were infiltrated with 10 μL of the highly virulent R. solanacearum strain FJC100301 suspension [optical density at 600 nm (OD₆₀₀) = 0.8] using a syringe without a needle, and the mock was inoculated with 10 mm MgCl₂. For exogenous phytohormone application, pepper plants at the four‐leaf stage were sprayed with 1 mm salicylic acid (SA), 100 µm methyl jasmonate (MeJA) or 100 µm ethephon (ETH), and the mock treatment was sprayed with a corresponding solvent or sterile double‐distilled H₂O. The leaves were collected at the indicated time points for RNA extraction and qRT‐PCR analysis. The relative transcript levels of CaLRR51 were compared with those in mock‐treated control plants. Data are the means ± standard deviation from three independent experiments. Asterisks indicate statistically significant differences compared with mock treatment by the least‐significant difference (LSD) test (*P < 0.05; **P < 0.01).