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. 2016 Sep 20;18(8):1089–1100. doi: 10.1111/mpp.12462

Figure 3.

Figure 3

Virus‐induced gene silencing (VIGS) of CaLRR51 enhances the susceptibility of pepper to Ralstonia solanacearum. (A) Relative transcriptional expression of CaLRR51 in leaves of CaLRR51‐silenced pepper plants (TRV:CaLRR51) versus empty vector control plants (TRV:00) by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) at 24 and 48 h post‐inoculation (hpi) with R. solanacearum or MgCl2 (mock). (B) Bacterial growth (colony‐forming units, cfu) in CaLRR51‐silenced or empty vector control pepper leaves inoculated with R. solanacearum at 2 and 4 days post‐inoculation (dpi). Data are the means ± standard deviation of three independent experiments. Different letters indicate statistically significant differences compared with the empty vector control plants by the least‐significant difference (LSD) test (P < 0.01). (C) Disease symptoms of CaLRR51‐silenced and empty vector control plants at 7 dpi after root inoculation with R. solanacearum.