Table 1.
Primers used in this study.
| Primer | Primer sequence | Comments |
|---|---|---|
| SsFkh1‐F | CCATCTTCCGGTAAGAGGGCCC | SsFkh1 (Forward) |
| SsFkh1‐R | ATAACTTACTTGCCAACCGTGGG | SsFkh1 (Reverse) |
| pSD‐1 | CTCTAGAGACGTCGAGAACCAATGATGAA(XbaI) | SsFkh1 (Forward) |
| pSD‐2 | CTCTAGAGCGAACATCATCTCAACTCCA(XbaI) | SsFkh1 (Reverse) |
| pSl‐L‐1 | CCTCGAGGACGTCGAGAACCAATGATGAA(XhoI) | SsFkh1 (Forward) |
| pSl‐L‐2 | CAAGCTTGCGAACATCATCTCAACTCCA(HindIII) | SsFkh1 (Reverse) |
| pSl‐R‐1 | GGCATGCCACGTCGAGAACCAATGATGAA(SphI) | SsFkh1 (Forward) |
| pSl‐R‐2 | CAGATCTGCGAACATCATCTCAACTCCA(BglII) | SsFkh1 (Reverse) |
| gene‐F | TGTCCGGTGCCCTGAATGAACT | Geneticin (Forward) |
| gene‐R | GCCGCCAAGCTCTTCAGCAATAT | Geneticin (Reverse) |
| hyg‐F | CGTCTGCTGCTCCATACAAG | Hygromycin (Forward) |
| hyg‐R | GCGAAGAATCTCGTGCTTTC | Hygromycin (Reverse) |
| qSsFkh1‐F | CCTCAAATCAACTGCCACGC | RT‐PCR for SsFkh1 (Forward) |
| qSsFkh1‐R | AGAGTGACGCCCAACATTGA | RT‐PCR for SsFkh1 (Reverse) |
| Actin‐F | GAATGTGTAAGGCCGGTTTCGC | Actin (Forward) |
| Actin‐R | CATCCCAGTTGGTGACGACACC | Actin (Reverse) |
| qSsPks1‐F | ACTGCTACGCCGAAACCATC | RT‐PCR for SsPks1 (Forward) |
| qSsPks1‐R | CGCATAGGACCTGCCAACTC | RT‐PCR for SsPks1 (Reverse) |
| qSsLac1‐F | ACCGCAGTCGCTTCTCAATA | RT‐PCR for SsLac1 (Forward) |
| qSsLac1‐R | TGCATGATCGGAGGGAACAC | RT‐PCR for SsLac1 (Reverse) |
| qSsLac2‐F | ACACTGTGAGAGCGGAATGG | RT‐PCR for SsLac2 (Forward) |
| qSsLac2‐R | CGACGTAGTGCTAGATCCCG | RT‐PCR for SsLac2 (Reverse) |
| qSsLac3‐F | ACCACACCGTTACCCAATCC | RT‐PCR for SsLac3 (Forward) |
| qSsLac3‐R | ACCACATTGGTGTAGCGACC | RT‐PCR for SsLac3 (Reverse) |
Italic indicates the restriction enzyme digestion sites.