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. 2016 Jul 26;18(4):582–595. doi: 10.1111/mpp.12419

Figure 1.

Figure 1

Mitogen‐activated protein kinase (MAPK) activation induced by oligogalacturonides (OGs) is partially affected only in AvrPto‐expressing line 4.1, but not in line 5.1 and the bak1‐5 bkk1‐1 double mutant. Levels of phosphorylated MPK3 and MPK6 (pMPK3 and pMPK6) in seedlings of wild‐type (Col‐0), AvrPto‐expressing lines (#4.1 and #5.1) and bak1‐5 bkk1‐1 after elicitation with OGs (40 µg/mL) or flg22 at the indicated time points were determined by immunoblot analysis using an anti‐p44/42‐ERK antibody (top panels). Levels of MPK3 and MPK6 total proteins were determined using specific antibodies (bottom panels). The identity of individual MAPKs, as determined by size, is indicated by arrows. Experiments were repeated three times with similar results.