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. 2016 Jul 26;18(4):582–595. doi: 10.1111/mpp.12419

Figure 2.

Figure 2

Reactive oxygen species (ROS) production and callose deposition in response to oligogalacturonides (OGs) are impaired in AvrPto‐expressing and bak1‐5 bkk1‐1 double mutant plants. (a) ROS production, expressed in relative light units (RLUs), was measured in leaf discs of wild‐type (Col‐0), AvrPto‐expressing lines (#4.1 and #5.1) and bak1‐5 bkk1‐1 mutant plants during elicitation with water, OGs or flg22. Results are the average ± standard error (SE) (n = 12). (b) Callose deposition visualized by aniline blue staining in leaves of Col‐0, AvrPto‐expressing lines (#4.1 and #5.1) and bak1‐5 bkk1‐1 mutant plants 24 h after syringe infiltration with water, OGs or flg22. All images are at the same scale (scale bar, 0.2 mm; 10× magnification). The average number of callose deposits (± SE) of 10 different leaf samples for each treatment is indicated in the images. Experiments in (a) and (b) were repeated three times with similar results.