Figure 1.

Expression analysis of MoYCP4 in the ΔMoap1 mutant and the DNA‐binding activity of MoAP1. (A) Transcriptional levels of MoYCP4 in the wild‐type and ΔMoap1 mutant before and after exposure to H₂O₂ measured by quantitative real‐time polymerase chain reaction (qRT‐PCR). (B) The digoxigenin (DIG)‐labelled promoter DNA of MoYCP4 was incubated in the absence or presence of purified MoAp1 protein. Proteinase K was added after the incubation of MoAp1 with the probe, and the sample was incubated for 10 min at room temperature. GST, glutathione S‐transferase. Double asterisks represent significant differences (P < 0.01).