Infectious hyphal growth on barley leaves. (A) Excised barley leaves from 7‐day‐old barley seedlings were inoculated with conidial suspension (5 × 104 spores/mL). Infectious growth was observed at 24 h post‐inoculation (hpi). (B) Statistical analysis of each type of infectious hyphal shape for each tested strain; 100 infecting hyphae were counted per replicate and the experiment was repeated three times. AP, appressorium; IH, infected hypha. Grade 1, no penetration; grade 2, with a penetration peg; grade 3, with a single invasive hypha; grade 4, with extensive hyphal growth.