Figure 7.

The Poa semilatent virus (PSLV) TGB2 and TGB3 proteins are unable to functionally replace the Hibiscus green spot virus (HGSV) BMB2 protein. (A) Complementation of cell‐to‐cell movement of PVX‐POL‐GFP by combinations of BMB1 with BMB2, TGB3 and TGB2 + TGB3. The leaf was imaged under UV light at 4 days post‐infiltration (dpi). Broken lines encircle infiltrated areas. (B) Co‐expression of GFP‐BMB1 with PSLV TGB3. (C) Co‐expression of GFP‐BMB1 with PSLV TGB2 and TGB3. Images in (B) are reconstructed from Z‐series of optical sections. Scale bars: (B, C) 20 μm. BMB, binary movement block; GFP, green fluorescent protein; PVX, Potato virus X; TGB, triple gene block.