Figure 4.

Perturbation of jasmonic acid (JA) signalling has no detectable effect on plant susceptibility. (a) Three‐week‐old wild‐type Castlemart, def1 and 35S::prosystemin tomato plants inoculated with water (mock), Fol007 or FolΔAvr2 at 21 days post‐infection (dpi). Disease symptoms were scored by measuring the fresh weight (b) and disease index (range, 0–4) (c) of 20 plants per treatment/genotype combination. Circle, × and Δ indicate Castlemart, def1 and 35S::prosystemin, respectively. The bioassay was repeated three times with similar results. (d) Representative stem sections taken from the cotyledon node (top left), second node (top right) and fourth node (bottom) of individual treated plants (n = 6) after incubation for 5 days on potato dextrose agar (PDA) plates. (e) Colonization is expressed as the percentage of infected slices of all stem pieces (n = 6). The experiment was repeated twice with similar results. (f) Transcription patterns of proteinase inhibitor I (PI‐I) in Fol007‐inoculated wild‐type Castlemart and def1 at 0, 3, 7 and 14 dpi. Three biological replicates for each line were analysed. The data are expressed as the mean ± standard deviation (SD). The experiment was performed twice with similar results.