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AVR‐Pia expression in a penetration‐deficient Magnaporthe oryzae mutant. Rice sheath assays of Δpls1‐PPR::eGFP (A) and Δpls1‐PPR::AVR‐Pia::4GGS::eGFP (C) were conducted at 24 h post‐inoculation (hpi), and fluorescence observation of liquid culture of Δpls1‐PPR::eGFP (B) and Δpls1‐PPR::AVR‐Pia::4GGS::eGFP (D) was performed at 24 hpi. AP, appressorium; GFP, green fluorescence protein; white arrow, tip of hypha. Scale bars, 5 μm.
