Figure 6.

Loss of Lip2 alters the cardiolipin (CL) composition of Ustilago maydis cells. CL profiles were obtained for the wild‐type (wt) strain and the Δlip2 mutant by liquid chromatography‐mass spectrometry (LC‐MS) analysis. The predicted compositions of the CLs are labelled below the graph based on the predicted acyl chains and double bonds (e.g. 72:7). For the two most abundant species, 72:7 is predicted to have three linoleic acid chains (18:2) and one oleic acid chain (18:1), whereas the 72:8 species is predicted to have four linoleic acid chains. The percentage of each of the indicated CL species is also indicated below the graph. The analysis was repeated with multiple injections. Injections were analysed in the (M + H)⁺ and (M + NH₄)⁺ modes, and the average of the technical replicates was calculated for each mode and each biological replicate. The overall averages for the wt strain and the mutant and the standard deviations are shown. A t‐test was used to identify significant differences between wt (filled bars) and mutant (open bars) (**P < 0.01).