Figure 1.

Transcript levels of SlPLC gene family, SA‐ and JA‐ defense gene markers and BcACT during the interaction between tomato and B. cinerea. Detached leaves from 6 weeks old tomato plants were droplet inoculated with B. cinerea isolate B05.10 spore suspension (10⁶ spores.mL⁻¹). Leaflets were harvested at the indicated hours post‐inoculation (hpi). Total RNA was isolated and transcript levels of SlPLC1 – SlPLC6 genes (A), SA‐defense gene markers SlPR1a and SlPR2b and JA‐defense gene markers SlPI‐I and SlPI‐II (B), and BcACT (C), were determined by RT‐qPCR. Transcript levels were normalized to SlACT. Error bars represent standard errors of three independent experiments. Asterisks denote that means are significantly different from 0 hpi samples according to a t‐test (P < 0.05).