Figure 2.

Specificity of virus‐induced gene silencing of SlPLC2 on tomato. Fourteen days old tomato seedlings were agroinfiltrated with the tobacco rattle virus (TRV) silencing constructs TRV:GUS (control) or TRV:SlPLC2. After 4 weeks, detached leaves were droplet inoculated with B. cinerea isolate B05.10 spore suspension (10⁶ spores.mL⁻¹). Leaflets were harvested at the indicated hours post‐inoculation (hpi). Total RNA was isolated and transcript levels of the six SlPLC genes were determined by RT‐qPCR. Transcript levels were normalized to SlACT. Error bars represent standard errors of three independent experiments. Asterisks denote that means are significantly different from inoculated TRV:GUS samples according to a t‐test (P < 0.05).