Figure 4.

Production of H₂O₂ on SlPLC2 silenced tomato plants inoculated with B. cinerea. Fourteen days old tomato seedlings were agroinfiltrated with the constructs TRV:GUS (control) or TRV:SlPLC2. After 4 weeks, detached leaves were spray inoculated with B. cinerea isolate B05.10 spore suspension (10⁶ spores.mL⁻¹). Leaflets were harvested at 24 hpi and H₂O₂ production was detected by 3,3‐diaminobenzidine (DAB) staining immediately after harvesting. (A) DAB stained tissue was macroscopically observed. Pictures were taken from representative leaflets (scale bars=1.5 mm). (B) Quantification of DAB stained area was performed using ImageJ 1.3 software and expressed as a percentage of inoculated TRV:GUS samples which was set to 100%. Error bars represent standard errors of three independent experiments. Asterisks denote that means are significantly different according to a t‐test (P < 0.001).