Figure 2.

Overexpression of MoMCK 1 and constitutive activation of MoMps1 (import of a MKK 1 ^T369D,T375D construct) in the ΔMopdeH mutant suppress the defects of hyphal autolysis and pathogenicity. (A) Autolysis observation. ΔMopdeH mutant showed apparent autolysis of mycelia on complete medium (CM) after culture for 2 weeks (top). Overexpression of MoMCK 1 and constitutive activation of MoMps1 strains delayed the process of autolysis (bottom). (B) Rice spraying assay. Four millilitres of conidial suspension (5 × 10⁴ spores/mL) of each strain were sprayed onto 2‐week‐old rice seedlings. Diseased leaves were photographed after 7 days. (C) Observation of infectious capacity on barley. Excised barley leaves from 7‐day‐old barley seedlings were inoculated with conidial suspension (5 × 10⁴ spores/mL) of each strain. Infectious growth was observed at 24 h after inoculation. Overexpression of MoMCK 1 and constitutive activation of MoMps1 in ΔMopdeH strains partially restored the infection defect of the ΔMopdeH mutant.