Figure 2.

NS_M‐dependent and NS_M‐independent hypersensitive cell death response (HR) induction by Sw‐5 orthologues. (A) Analysis of HR induction by Sw‐5a and Sw‐5a^S was performed in a similar manner to the experiment described in Fig. 1 and using the functional Sw‐5b as a positive control. In the first column of (square) images, Nicotiana benthamiana leaves were co‐infiltrated with agrobacteria harbouring Sw‐5a, Sw‐5b or Sw‐5a^S (cloned into pK7WGF2) and p19 for cell death triggering by auto‐activation. In the other columns, images are shown from leaf areas co‐infiltrated with agrobacteria harbouring the same Sw‐5 constructs and BR‐01‐NS_M (second column) or GRAU‐NS_M (third column), all constructs cloned in pK2GW7. Images were taken at 4 days post‐agroinfiltration (dpa). (B) Western immunoblot detection of Sw‐5a^S in leaves from (A) (first column) co‐infiltrated with pK7WGF2 constructs of Sw‐5a^S and p19. Western blot was screened with anti‐green fluorescent protein (anti‐GFP) as primary antibody.