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. 2014 Aug 24;16(2):188–200. doi: 10.1111/mpp.12172

Figure 2.

figure

Pronase treatment and size determination of the necrotrophic effector PttNE1. (A) The reaction of Hector to infiltration with intercellular wash fluid (IWF) and the same IWF treated with Pronase for 2 h. (B) The reaction of Hector to infiltration with the IWF and fractions 17–22 from size‐exclusion high‐pressure liquid chromatography (HPLC). Necrotic centres on leaves are tissue damage from the infiltration. Sensitivity to PttNE1 is identified on the basis of tan necrosis between the infiltration point and the black marked end of the infiltrated area (i.e. fractions 19 and 20).