Pseudomonas corrugata strain CFBP 5454 and derivative mutant strain PCONRPS and GLRFIA population concentrations in Nicotiana benthamiana leaves Three N. benthamiana leaf discs were collected independently and processed at each time point (0, 1, 2, 3 and 4 days post‐inoculation). In this trial, Pseudomonas syringae pv. tabaci strain DAPP‐PG632 was used as the pathogenic control strain. In both trials, samples were homogenized with 1 mL of distilled water; serial dilutions were plated onto NDA medium (nutrient agar plus 1% dextrose) plus antibiotics as described in Experimental procedures. Values are means ± standard error (SE) of multiple replicates. The results presented are representative of two independent experiments.