Figure 1.

Schematic representation of the constructs. The PVA‐SC6K construct allows the expression of 2×Strep‐Cerulean fluorescent protein (CFP)‐6K2 (SC6K2) fusion protein in the context of Potato virus A (PVA) infection. The PVA‐C6K construct is similar, except that it lacks the 2×Strep‐tag. SC6K2 fusion is expressed from the MC‐SC6K construct (MC, membrane control) in a non‐infected background. The PVA‐6KY construct allows the expression of the 6K2‐yellow fluorescent protein (6KY) fusion in the context of PVA infection. Hatched rectangles flanking the fluorescent protein‐6K2 cassettes denote NIa protease cleavage sites.