Table 2.
Proteome of affinity‐purified PVA 6K2‐induced vesicles, location‐ and function‐based classification.
| Category | TAIR ID | N. benthamiana ID*(solgenomics.net) | Name† | Σ Unique peptides | Σ Peptides | Σ PSM | Σ Coverage |
|---|---|---|---|---|---|---|---|
| Chaperone | |||||||
| AT5G02500 | NbS00025223g0018.1 | HSC70‐1 | 6 | 18 | 95 | 42.47 | |
| AT5G02500 | NbS00016136g0003.1 | HSC70‐1 | 3 | 17 | 92 | 39.59 | |
| AT3G12580 | NbS00009983g0008.1 | HSP70 | 2 | 15 | 89 | 51.82 | |
| AT5G50920 | NbS00017400g0004.1 | ATP‐dependent Clp protease ATP‐binding subunit clpA homologue CD4B, chloroplastic | 2 | 26 | 55 | 38.15 | |
| AT5G28540 | NbS00040865g0006.1 | Luminal‐binding protein 5 | 6 | 8 | 26 | 14.81 | |
| AT5G56030 | NbS00025260g0001.1 | HSP81‐2 | 3 | 10 | 22 | 23.7 | |
| AT5G56030 | NbS00021897g0010.1 | HSP81‐2 | 4 | 11 | 21 | 18.99 | |
| AT4G24280 | NbS00052944g0006.1 | Chloroplast heat shock protein 70‐1 | 10 | 10 | 18 | 17.47 | |
| AT5G22060 | NbS00016695g0014.1 | DNAJ homologue 2 | 3 | 8 | 18 | 28.27 | |
| AT3G48870 | NbC25340200g0001.1 | ATP‐dependent Clp protease ATP‐binding subunit clpA homologue CD4B, chloroplastic | 2 | 7 | 17 | 64.43 | |
| AT5G22060 | NbS00004100g0004.1 | DNAJ homologue 2 | 2 | 7 | 15 | 22.15 | |
| AT3G13470 | NbS00034791g0001.1 | Chaperonin 60 subunit beta 2, chloroplastic | 4 | 4 | 8 | 9.05 | |
| AT4G24190 | NbS00013845g0026.1 | Heat shock protein 90 | 4 | 4 | 5 | 5.65 | |
| Chloroplast | |||||||
| AT2G39730 | NbS00047700g0013.1 | Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic | 2 | 15 | 62 | 43.53 | |
| AT1G06950 | NbS00009678g0004.1 | Protein TIC110, chloroplastic | 9 | 23 | 59 | 32.34 | |
| AT2G39730 | NbS00009714g0011.1 | Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic | 2 | 14 | 56 | 47.8 | |
| AT3G01500 | NbS00016159g0006.1 | Carbonic anhydrase, chloroplastic | 3 | 10 | 53 | 43.71 | |
| AT3G46740 | NbS00002677g0011.1 | Protein TOC75‐3 | 14 | 14 | 50 | 30.02 | |
| AT1G06950 | NbS00021174g0005.1 | Protein TIC110, chloroplastic | 3 | 17 | 38 | 23.96 | |
| AT4G02510 | NbS00024250g0002.1 | Translocase of chloroplast 159, chloroplastic | 13 | 13 | 30 | 16.47 | |
| AT3G47520 | NbS00008675g0002.1 | Malate dehydrogenase, chloroplastic | 5 | 8 | 18 | 29.25 | |
| AT5G38410 | NbS00022486g0001.1 | Ribulose bisphosphate carboxylase small chain 8B, chloroplastic | 5 | 6 | 18 | 44.75 | |
| AT2G20260 | NbS00003075g0011.1 | Photosystem I reaction centre subunit IV B, chloroplastic | 2 | 6 | 18 | 53.15 | |
| AT2G24820 | NbS00029739g0004.1 | Translocon at the inner envelope membrane of chloroplasts 55‐II | 2 | 5 | 16 | 18.2 | |
| AT3G56940 | NbS00020307g0015.1 | Magnesium‐protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic protein, putative (Crd1) | 7 | 7 | 15 | 19.55 | |
| AT5G16620 | NbS00017610g0108.1 | Translocon at the inner envelope membrane of chloroplasts 40 | 3 | 5 | 15 | 15.41 | |
| AT1G61520 | NbS00021892g0003.1 | Chlorophyll a/b‐binding protein 8 | 3 | 4 | 13 | 30.56 | |
| AT1G74470 | NbS00056940g0001.1 | Geranylgeranyl diphosphate reductase, chloroplastic | 5 | 5 | 12 | 14.22 | |
| AT5G05000 | NbS00005332g0008.1 | Translocon at the outer envelope membrane of chloroplasts 34 | 4 | 4 | 12 | 25.58 | |
| AT4G23430 | NbS00010186g0028.1 | Short‐chain dehydrogenase TIC 32 | 4 | 4 | 11 | 25.08 | |
| AT2G16640 | NbS00010608g0012.1 | Translocase of chloroplast 132, chloroplastic | 3 | 7 | 10 | 5.77 | |
| AT2G28000 | NbS00033391g0006.1 | RuBisCO large subunit‐binding protein subunit alpha, chloroplastic | 4 | 4 | 10 | 11.29 | |
| AT2G16640 | NbS00004829g0016.1 | Translocase of chloroplast 132, chloroplastic | 2 | 6 | 9 | 5.17 | |
| AT2G38040 | NbS00026142g0005.1 | Acetyl‐coenzyme A carboxylase carboxyl transferase subunit alpha, chloroplastic | 4 | 5 | 8 | 12.61 | |
| AT1G77590 | NbS00027106g0001.1 | LACS9, long‐chain acyl‐CoA synthetase 9, chloroplastic | 4 | 4 | 8 | 9.95 | |
| AT5G16620 | NbS00033277g0017.1 | Protein TIC 40, chloroplastic | 2 | 4 | 8 | 20.29 | |
| AT2G05100 | NbS00019403g0016.1 | Chlorophyll a/b‐binding protein 37, chloroplastic | 3 | 4 | 7 | 22.26 | |
| Cytoplasm | |||||||
| AT1G62020 | NbS00031319g0001.1 | Coatomer subunit alpha‐1 | 2 | 4 | 8 | 4.75 | |
| Cytoskeleton | |||||||
| AT1G75780 | NbS00056603g0002.1 | Tubulin beta‐1 chain | 2 | 12 | 49 | 39.39 | |
| AT5G23860 | NbS00001148g0004.1 | Tubulin beta‐8 chain | 1 | 9 | 44 | 30.72 | |
| AT4G14960 | NbS00001594g0015.1 | Tubulin alpha‐6 chain | 9 | 9 | 27 | 32.55 | |
| AT5G12250 | NbS00023211g0007.1 | Tubulin beta‐6 chain | 2 | 8 | 19 | 30.15 | |
| Metabolic enzyme | |||||||
| AT1G12900 | NbS00001169g0153.1 | Glyceraldehyde 3‐phosphate dehydrogenase A subunit 2 | 10 | 12 | 59 | 38.42 | |
| AT4G35000 | NbS00062043g0007.1 | l‐Ascorbate peroxidase 3, peroxisomal | 4 | 10 | 31 | 37.09 | |
| AT3G27820 | NbS00023324g0005.1 | Probable monodehydroascorbate reductase, cytoplasmic isoform | 10 | 10 | 30 | 23.81 | |
| AT4G35000 | NbS00035202g0004.1 | l‐Ascorbate peroxidase 3, peroxisomal | 2 | 7 | 26 | 37.77 | |
| AT3G16950 | NbS00014159g0003.1 | Dihydrolipoyl dehydrogenase | 4 | 8 | 21 | 19.07 | |
| AT2G34590 | NbS00007894g0004.1 | Pyruvate dehydrogenase E1 component subunit beta | 6 | 6 | 17 | 22.74 | |
| AT1G01090 | NbS00017334g0012.1 | Pyruvate dehydrogenase E1 component subunit alpha | 4 | 6 | 15 | 20.29 | |
| AT1G01090 | NbS00034265g0006.1 | Pyruvate dehydrogenase E1 component subunit alpha | 4 | 6 | 12 | 17.45 | |
| AT2G22780 | NbS00002298g0008.1 | Malate dehydrogenase, glyoxysomal | 5 | 5 | 11 | 16.87 | |
| AT3G14420 | NbS00005125g0015.1 | Peroxisomal (S)−2‐hydroxy‐acid oxidase | 6 | 6 | 8 | 24.44 | |
| AT4G35090 | NbS00006116g0019.1 | Catalase isozyme 1 | 5 | 5 | 8 | 17.97 | |
| AT1G44170 | NbS00047628g0009.1 | Aldehyde dehydrogenase | 4 | 4 | 7 | 10.48 | |
| Nucleotide binding | |||||||
| AT4G17170 | NbS00004361g0010.1 | Ras‐related protein RABB1c | 6 | 6 | 19 | 37.44 | |
| AT5G27540 | NbS00036282g0010.1 | MIRO‐related GTP‐ase 1 | 4 | 11 | 17 | 28.59 | |
| AT2G27600 | NbS00006021g0010.1 | AAA‐type ATPase family protein | | 8 | 8 | 17 | 31.34 | |
| AT1G78900 | NbS00033958g0004.1 | V‐type proton ATPase catalytic subunit A | 6 | 6 | 13 | 14.36 | |
| Ribosome/protein biosynthesis | |||||||
| AT4G10450 | NbS00002134g0112.1 | Ribosomal protein L6 family | 2 | 11 | 34 | 61.47 | |
| AT2G27710 | NbS00029619g0005.1 | 60S acidic ribosomal protein P2 | 3 | 5 | 13 | 42.95 | |
| AT2G19730 | NbS00054743g0008.1 | Ribosomal L28e protein family | 5 | 6 | 12 | 27.68 | |
| AT4G09800 | NbS00056355g0003.1 | S18 ribosomal protein | 2 | 4 | 12 | 32.24 | |
| AT1G61580 | NbS00018918g0016.1 | R‐protein L3 B | 3 | 7 | 11 | 18.77 | |
| AT3G13920 | NbS00044851g0011.1 | Eukaryotic translation initiation factor 4A1 | 5 | 5 | 10 | 15.98 | |
| AT1G48830 | NbS00039588g0004.1 | Ribosomal protein S7e family protein | 4 | 4 | 10 | 30.96 | |
| AT1G61580 | NbS00045996g0004.1 | 60S ribosomal protein L3 | 3 | 7 | 9 | 18.77 | |
| AT3G49910 | NbS00036380g0001.1 | 60S ribosomal protein L26‐1 | 2 | 5 | 9 | 32.19 | |
| AT1G57720 | NbS00006811g0211.1 | Probable elongation factor 1‐gamma 2 | 4 | 4 | 9 | 9.38 | |
| AT3G11250 | NbS00018504g0008.1 | 60S acidic ribosomal protein P0 | 5 | 5 | 8 | 30.87 | |
| Other | |||||||
| AT1G04690 | NbS00043430g0001.1 | Probable voltage‐gated potassium channel subunit beta; KAB1, KV‐BETA1 | 13 | 13 | 71 | 64.75 | |
| AT1G34430 | NbC26077604g0003.1 | Dihydrolipoyllysine‐residue acetyltransferase component of pyruvate dehydrogenase complex | 2 | 8 | 26 | 30.49 | |
| AT5G08540 | NbS00000164g0018.1 | Unknown protein | 9 | 9 | 21 | 34.58 | |
| AT3G13930 | NbS00000479g0101.1 | Dihydrolipoyllysine‐residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial | 8 | 8 | 19 | 19.78 | |
| AT1G11910 | NbS00028367g0004.1 | Aspartic proteinase A1 | 2 | 6 | 19 | 17.98 | |
| AT5G24650 | NbS00016191g0009.1 | Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein | 6 | 6 | 18 | 26.92 | |
| AT1G11910 | NbS00031482g0006.1 | Aspartic proteinase A1 | 2 | 6 | 17 | 17.39 | |
| AT3G01280 | NbS00027615g0006.1 | Voltage‐dependent anion channel 1 | 2 | 4 | 17 | 23.91 | |
| AT5G12470 | NbS00005727g0008.1 | Unknown | 6 | 7 | 15 | 26.77 | |
| AT1G17860 | NbC24872723g0001.1 | Kunitz family trypsin and protease inhibitor protein | 5 | 5 | 15 | 35.85 | |
| AT1G05270 | NbS00011400g0014.1 | TraB family protein | 3 | 7 | 14 | 25.92 | |
| AT2G38040 | NbS00048878g0013.1 | CAC3, acetyl Co‐enzyme A carboxylase carboxyltransferase alpha subunit | 9 | 10 | 13 | 16.33 | |
| AT5G22640 | NbS00044837g0004.1 | emb1211, MORN (Membrane Occupation and Recognition Nexus) repeat‐containing protein | 7 | 7 | 13 | 14.47 | |
| AT1G33970 | NbS00011570g0012.1 | P‐loop‐containing nucleoside triphosphate hydrolases superfamily protein | 5 | 5 | 13 | 13.86 | |
| AT2G28900 | NbS00045823g0014.1 | Outer plastid envelope protein 16‐1 | 4 | 4 | 13 | 26.75 | |
| AT5G62810 | NbS00031622g0006.1 | Peroxisomal membrane protein PEX14 | 2 | 5 | 11 | 36.63 | |
| AT1G10510 | NbS00019582g0003.1 | emb2004, RNI‐like superfamily protein | 6 | 6 | 10 | 19.64 | |
| AT2G20580 | NbS00013228g0016.1 | 26S proteasome regulatory subunit S2 1A | 2 | 5 | 8 | 9.7 | |
| AT1G11910 | NbS00050653g0003.1 | Aspartic proteinase A1 | 4 | 4 | 8 | 11.42 | |
| AT2G17840 | NbS00004850g0017.1 | ERD7, senescence/dehydration‐associated protein‐related | 5 | 5 | 7 | 16.82 | |
| AT3G57090 | NbS00059906g0004.1 | BIGYIN, FIS1A; tetratricopeptide repeat (TPR)‐like superfamily protein | 4 | 4 | 7 | 27.97 | |
| AT5G62810 | NbS00005874g0019.1 | Peroxisomal membrane protein PEX14 | 3 | 4 | 7 | 18.94 | |
| AT2G44640 | NbS00028734g0022.1 | Uncharacterized protein | 4 | 4 | 6 | 13.8 | |
| AT4G36720 | NbS00015416g0013.1 | HVA22‐like protein K | 4 | 4 | 5 | 30.48 | |
| AT2G32730 | NbS00029577g0007.1 | 26S proteasome regulatory complex, non‐ATPase subcomplex, Rpn2/Psmd1 subunit | 4 | 4 | 5 | 8.39 | |
*Derived from the Nicotiana benthamiana annotated transcriptome list v0.4.4 (solgenomics.net).
†Derived from the N. benthamiana annotated transcriptome list v0.4.4 (solgenomics.net) or from the closest WU‐BLAST match in the TAIR web‐page.