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. 2015 May 8;17(1):29–41. doi: 10.1111/mpp.12263

Figure 5.

figure

Necrosis induction or suppression assays in Nicotiana benthamiana. (A, B) Areas of an N. benthamiana leaf infiltrated with Agrobacterium tumefaciens to transiently express RXLR18215, a suppressor of necrosis (B, black circle), or a pART27 empty vector control which does not suppress necrosis (A, black circle), and 24 h later infiltrated to express a necrosis‐inducing protein (PiNPP1.1; red circles in A and B), such that an overlapping region of infiltration is generated. (C) Number of times a candidate gene (RXLRs, CRNs and hypothetical proteins) suppressed cell death caused by PiNPP1.1 (results of at least 15 infiltrations per gene) in the transient expression assays, with those showing suppression activity 80% of the time or more highlighted in red. hai, hours after inoculation. Positive control SNE1 without a signal peptide (SP).