Figure 2.

diC14 induces local resistance to Pseudomonas syringae pv. tomato (Pst). (a) Leaves pre‐treated with HEPES (0.2 mm) (mock), diC14 or diC16 (20 μg/mL each) were inoculated 24 h later with Pst [10⁵ colony‐forming units (cfu)/mL] to quantify the bacterial content at 3 days post‐infection. Values represent mean ± standard deviation (SD) of two technical replicates. Similar results were obtained in two independent infection experiments. *Significant differences between mock and diC14 treatments (P < 0.05 by t‐test). (b) Cell death was evaluated by trypan blue staining on leaves sampled at 8 or 24 h post‐infiltration (hpi). The effects of mock solution (HEPES, 0.2 mm), diC14 (20 μg/mL) and Pst AvrRpm1 (10⁷ cfu/mL) are compared. Similar results were obtained in three independent experiments.