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. 2015 May 4;17(1):3–15. doi: 10.1111/mpp.12257

Figure 4.

figure

Expression of Dark‐inducible (DIN) homologues in Nicotiana benthamiana in response to Tobacco rattle virus (TRV) and Potato virus X (PVX). Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) analysis of NbNRIP1 (N receptor‐interacting protein 1), NbASN (Asparagine synthetase 1) and NbDIN11 expression in upper non‐inoculated leaves of plants infected with TRV‐GFP or PVX‐GFP. Accumulation levels are referenced to the mock‐inoculated controls that were arbitrarily assigned a value of unity after normalization to the NbACT internal control. The means of three measurements ± standard deviation (SD) are shown. Asterisks indicate statistical significance versus the mock‐inoculated control (P < 0.001, Duncan's multiple range test). GFP, green fluorescent protein.