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. 2015 May 4;17(1):3–15. doi: 10.1111/mpp.12257

Figure 6.

figure

Effect of AtDIN11 (Dark‐inducible 11) and NbASN (Asparagine synthetase 1) on the expression of defence‐related genes in Tobacco rattle virus (TRV)‐infected plants. Expression values, determined by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR), are the means of three measurements ± standard deviation (SD). Asterisks indicate statistical significance versus the controls (P < 0.001, Duncan's multiple range test). (A) Expression analysis of AtPR1 (Pathogenesis‐related 1) and AtPDF1.2 (Plant defensin 1.2) in upper non‐inoculated rosette leaves of Arabidopsis DIN11 RNA interference (RNAi) lines (L10 and L21) systemically infected with TRV. Values in infected Col‐0 plants were assigned a value of unity after AtCBP20 normalization. (B) Expression analysis of NbPR1 and NbHSP70 (Heat‐shock protein 70) in upper N. benthamiana leaves systemically infected with TRV‐NbASN relative to control plants infected with TRV‐GFP which were assigned a value of unity after NbACT normalization. (C) Expression analysis of NbPR1 and NbHSP70 in TRV‐infected N. benthamiana leaves that transiently overexpressed the Arabidopsis AtDIN6 homologue relative to the control leaves in which an empty vector (EV) was agroinoculated (this sample was assigned a value of unity after NbACT normalization).