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. 2015 Jan 29;16(7):699–709. doi: 10.1111/mpp.12228

Figure 3.

figure

Expression of representative AvrLm2 (avirulent) alleles during in vitro growth of Leptosphaeria maculans and oilseed rape infection. Expression of the AvrLm2 alleles was analysed in the avirulent isolate 00‐100 by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). 3–12, RT‐PCR products obtained from RNA isolated from oilseed rape cotyledons (Westar) at 3–12 days post‐infection; myc, RT‐PCR product obtained from RNA isolated from mycelial culture; sp Fries, RT‐PCR product obtained from RNA isolated from conidia germinating in rich medium (Fries). RNA extracted from uninfected cotyledons and water were used as negative controls. Gene expression levels are relative to actin. Each data point is the average of three biological repeats (extractions from different biological material). The standard error of the mean normalized expression level is indicated by error bars.