Table 3.
List of secreted proteins identified in a proteomic analysis of Sclerotinia sclerotiorum cell walls.
| Protein name and GI no. | PDB | Vogel's glucose | Sclerotia |
|---|---|---|---|
|
Cellulase (GH5) SS1G_00458 156064897 |
Yes (4) | Yes (4) | No |
|
Cellulase SS1G_04085 156056709 |
Yes (2) | Yes (2) | Yes (2) |
|
Cellulase SS1G_07554 156049937 |
Yes (1) | Yes (1) | Yes (1) |
|
Cellobiohydrolase (GH7) SS1G_04945 156055188 |
Yes (3) | Yes (4) | No |
|
Glucoamylase (GH15) SS1G_13809 156032806 |
Yes (2) | No | Yes (2) |
|
Invertase (GH32) SS1G_07184 156051554 |
Yes (5) | Yes (1) | No |
|
Maltase (GH31) SS1G_01083 156061938 |
Yes (2) | Yes (2) | Yes (2) |
|
Pectate lyase SS1G_09216 156045880 |
Yes (3) | Yes (1) | No |
|
Pectate lyase SS1G_07393 156051972 |
No | Yes (4) | Yes (8) |
|
Glycosyl hydrolase (GH2) (galactosidase, mannosidase or arabinofuranosidase) SS1G_01262 156062292 |
Yes (4) | Yes (3) | Yes (1) |
|
β‐Glucosidase (GH3) SS1G_13255 156034641 |
Yes (5) | Yes (1) | Yes (12) |
|
β‐Glucosidase (GH3) glycosyl hydrolase SS1G_05368 156056032 |
Yes (1) | No | No |
|
β‐Glucosidase (GH3) SS1G_07146 156051478 |
No | Yes (3) | Yes (4) |
|
Aspartyl protease SS1G_03941 156059856 |
Yes (4) | Yes (1) | No |
|
Aspartyl protease SS1G_11818 156039363 |
Yes (1) | Yes (3) | Yes (5) |
|
Secreted protease, chymotrypsin‐like SS1G_09169 156045786 |
Yes (14) | Yes (7) | No |
|
Catalase SS1G_01081 156061934 |
Yes (9) | No | Yes (2) |
|
Peroxidase‐like SS1G_02495 156060705 |
Yes (5) | No | Yes (2) |
|
Alkaline phosphatase SS1G_07639 156050107 |
Yes (1) | No | No |
List of secreted proteins. The first column lists each of the secreted proteins identified in the proteomic analysis, together with its identification number. The second, third and fourth columns show which sample(s) contained the proteins (PDB‐grown hyphae, Vogel's glucose‐grown hyphae and sclerotia), and the number of peptides that were identified in the cell wall samples. PDB, potato dextrose broth.