Table 1.
List of primer and probe sequences used to analyse the expression of defence‐associated genes in citrus. The assay names are based on homology to genes characterized in Arabidopsis. Loci of the respective genes are from the C itrus sinensis genomic database available at the National Center for Biotechnology Information (NCBI). Forward primer (f), reverse primer (r) and probe (p) sequences were generated using Primer Express Software 3.0 (Applied Biosystems, Foster City, CA, USA)
| Assay | Primer/probe | Citrus sinensis locus |
|---|---|---|
| 5.8S rRNA | f: CGACTCTCGGCAACGGATA | JQ990165 |
| r: CGCATTTCGCTACGTTCTTCA | ||
| p: CTCGGCTCTCGCATC | ||
| AZI1 | f: CCATCAAAGCGAACATTTTGG | LOC102617861 |
| r: CGTTCAAAAGAAGGCTGAGTGA | ||
| p: ATCAACCTTAATATCCC | ||
| CHI | f: GCCGGCTTCCGGGATAC | Z70032 |
| r: CTTGGCCACATTCAATTCCA | ||
| p: CTAACCACAAATATAATCAACG | ||
| COI1 | f: GGGAATGGAGGATGAAGAAGGT | LOC102620384 |
| r: GCCCTGAGCCAAAGCAATTA | ||
| p: TTGTCTCGCAAAGAGGA | ||
| EDR1 | f: TCCAGGAGTGCTTTGAGTGGTA | LOC102618775 |
| r: GCCCATTTAACTGACTTGTGCTAGA | ||
| p: TGGCCCATCATTGG | ||
| EDS1 | f: GGCTCGAGTATGCCCTGAAG | LOC102618041 |
| r: CTTGCCCAGAAACATGATTCC | ||
| p: ATCGGCAGGATCCAG | ||
| EDS5 | f: ATCGAAGTTTGGTAAAGGCAAGA | LOC102615350 |
| r: AGCGTGGATCCAATGAGAAGA | ||
| p: TGCTGCTGAAGTCAC | ||
| GST1 | f: GCCCGTTTGTCTCAGTCCAA | LOC102614737 |
| r: TGCAAATCGACCAAGGTGAA | ||
| p: ACTTGGCGTGCGACAG | ||
| ICS1 | f: CAGCGCTGGCCTTGGA | LOC102630235 |
| r: GGAGGTGGGTTGGATTTCAA | ||
| p: AAACTTCACTCTGCCATTT | ||
| JAR1 | f: AAGGCGATGCAGTCACAATG | LOC102611440 |
| r: TGGTGGAAATCAGGACCAAAG | ||
| p: AGCCCTGATGAAGTAA | ||
| NDR1 | f: GCGCCGACCGATCAGA | LOC102630232 |
| r: CGCCGACCCATTTGCA | ||
| p: TTTCCCGGGCGGTTT | ||
| NPR1 | f: CGTGGCATATTGTGATGCAAA | LOC102617188 |
| r: GTTGACATCAGCAAGTCCAAGATC | ||
| p: ACCACAACTGAGCTTC | ||
| NPR2 | f: ACCTTAGACGAAGCCAATGCA | LOC102621158 |
| r: CAGACAACACCTTGGGATCACA | ||
| p: TCCATTATGCTGCAGCGTA | ||
| NPR3 | f: TTTTATACTGGCCTTTCAGCATCA | LOC102624339 |
| r: CGTCTCAACTGTTTTAAGCAAAGC | ||
| p: TGCAAGCCAAGAGAC | ||
| PAL1 | f: CTCGGCCCTCAGATCGAA |
LOC102620173 LOC102620464 |
| r: CCGAGTTGATCTCCCGTTCA | ||
| p: TGATTCGGTTTGCAACCA | ||
| PBS1 | f: TCCAAAAGAACCAACTGCACAT | LOC102630702 |
| r: AGCAGCAAGCTCCCGAAAT | ||
| p: CCGCCCAAACGTTTA | ||
| PR1 | f: AAGGAAAGCGGATTGCAAACT | LOC102622841 |
| r: CTCGCCAAGCTTGAAATTGTC | ||
| p: CAGCATTCGTTCCCG | ||
| RAR1 | f: GTCAGAACGACGACGCTTTG | LOC102626257 |
| r: GCGTTGCAGCCAATTCG | ||
| p: AGCGTCTTCGATGCC | ||
| RdRp1 | f: CGGCAGCGCTTTATTGTTC | LOC102608479 |
| r: ACATCACCTGGATGCAAACAAG | ||
| p: ACTGGTGGTTGTTGCAA | ||
| SGT1 | f: GCTGATGCAGATGAGGACACA | LOC102623505 |
| r: CCCGTTTGACTCGACGAAAG | ||
| p: ACGAGCCATGAAAAA | ||
| WRKY22 | f: GCGGATTGTCTCGCATGTG | LOC102612567 |
| r: TTATGGGTTTCTGCCCGTATTT | ||
| p: AAGTGGGCTTGGCG |