Skip to main content
. 2016 Mar 15;17(8):1223–1236. doi: 10.1111/mpp.12359

Figure 4.

Figure 4

Differential PthA‐dependent induction of LOB (LATERAL ORGAN BOUNDARIES) and DIOX (citrus dioxygenase) genes in sweet orange and lemon plants. Expression levels of LOB1, LOB2, LOB3 and DIOX in ‘Pera’ and ‘Tahiti’ leaves infiltrated with the wild‐type (WT) Xanthomonas citri or the single pthA‐deletion mutants (Δ1, Δ3 and Δ4), 72 h post‐inoculation. (A) PthAs 1, 3 and 4 are required for LOB1 induction in lemon, whereas only PthA4 is important for LOB1 induction in ‘Pera’ sweet orange. However, PthA1 seems to repress LOB1 transcription in this host. (B, C) LOB2 and LOB3 are induced by X. citri in ‘Pera’ and ‘Tahiti’ plants; however, PthAs 1, 3 and 4 do not alter significantly LOB2 expression in ‘Tahiti’ or LOB3 expression in ‘Pera’ plants. LOB2 expression in ‘Pera’ sweet orange appears to require PthA1, but not PthA3 or PthA4. (D) The DIOX gene is also induced by X. citri WT and respective single pthA mutants in ‘Pera’ and ‘Tahiti’ leaves; however, deletion in pthA 1, 3 or 4 significantly reduces DIOX expression in ‘Pera’, but not in ‘Tahiti’, leaves. The error bars denote standard deviations, whereas the asterisks above the bars indicate statistically significant differences between X. citri and mutant‐inoculated plants (P < 0.05).