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. 2015 Aug 15;17(3):398–411. doi: 10.1111/mpp.12288

Figure 1.

figure

Translocation assay for predicted Xanthomonas euvesicatoria (X cv) type III effectors. (A) Suspensions [5 × 107 colony‐forming units (CFU)/mL] of X cv 85‐10 hrp G *ΔavrBs2avr B s2) or X cv 85‐10 hrp G * ΔhrpFhrp F) strains containing AvrBs262–574::HA (Empty), the indicated candidate effectors fused to AvrBs262–574::HA or AvrBs21–61 fused to AvrBs262–574::HA were infiltrated into ECW20R (carrying the B s2 resistance gene) or ECW30R (lacking the B s2 resistance gene) pepper leaves. Infected leaves were monitored for the appearance of cell death, washed in bleaching solution and photographed at 36 h after infection. (B) Total protein was extracted from overnight cultures of X cv 85‐10 hrp G * Δavr B s2avr B s2) or X cv 85‐10 hrp G * Δhrp Fhrp F) strains containing AvrBs262–574::HA (Empty), the indicated effectors fused to AvrBs262–574::HA or AvrBs21–61 fused to AvrBs262–574::HA, and analysed by Western blot analysis using anti:HA‐specific antibodies. HA, haemagglutinin.