Translocation assay for predicted Xanthomonas euvesicatoria (X
cv) type III effectors. (A) Suspensions [5 × 107 colony‐forming units (CFU)/mL] of X
cv 85‐10 hrp
G
*ΔavrBs2 (Δavr
B
s2) or X
cv 85‐10 hrp
G
* ΔhrpF (Δhrp
F) strains containing AvrBs262–574::HA (Empty), the indicated candidate effectors fused to AvrBs262–574::HA or AvrBs21–61 fused to AvrBs262–574::HA were infiltrated into ECW20R (carrying the B
s2 resistance gene) or ECW30R (lacking the B
s2 resistance gene) pepper leaves. Infected leaves were monitored for the appearance of cell death, washed in bleaching solution and photographed at 36 h after infection. (B) Total protein was extracted from overnight cultures of X
cv 85‐10 hrp
G
* Δavr
B
s2 (Δavr
B
s2) or X
cv 85‐10 hrp
G
* Δhrp
F (Δhrp
F) strains containing AvrBs262–574::HA (Empty), the indicated effectors fused to AvrBs262–574::HA or AvrBs21–61 fused to AvrBs262–574::HA, and analysed by Western blot analysis using anti:HA‐specific antibodies. HA, haemagglutinin.