Figure 5.

Development of disease symptoms in pepper plants inoculated with the X cv 85‐10 xop AP:Gn^R mutant. Pepper ECW30R plants were syringe infiltrated with a mock solution or with suspensions [5 × 10⁶ colony‐forming units (CFU)/mL] of X cv 85–10, wild‐type, mutated in xop AP (X cv xopAP:Gn^R) or mutated in xop AP and complemented with a plasmid carrying the xop AP gene. Inoculated areas were monitored for the appearance of disease symptoms and photographed at 6 days post‐inoculation (dpi) (A). Cell death (B) and leaf bleaching (C) in inoculated areas were quantified at different dpi by measuring electrolyte leakage and chlorophyll content, respectively. Values are the means ± standard error (SE) of five independent leaf areas. Asterisks indicate a significant difference (Student's t‐test, P < 0.05) relative to the X cv 85‐10 wild‐type treatment. The experiment was repeated three times with similar results.