Skip to main content
. 2015 Jun 7;17(2):173–183. doi: 10.1111/mpp.12270

Figure 2.

figure

Symptom development and viral accumulation in plants infected with Cucumber mosaic virus (CMV) and CMV‐2blm. (A) CMV‐2blm only induced severe disease symptoms in plants harbouring RDR6‐deficient mutations, including rdr6, rdr1/6, rdr2/6 and rdr1/2/6, but not in wild‐type, rdr1, rdr2 and rdr1/2 plants. Seedlings were photographed at 21 days after inoculation with purified CMV or CMV‐2blm viruses at a concentration of 20 μg/mL. (B) Accumulation of viral genomic RNA in systemically infected leaves of wild‐type and mutant plants infected with CMV or CMV‐2blm. Methylene blue‐stained rRNA was used as a loading control. It should be noted that 5 μg and 2 μg of total RNAs were used for the Northern blot analyses of CMV‐2blm and wild‐type CMV viral RNAs. wt, wild‐type.