Figure 4.

Transgenic expression of 2b, but not 2blm, affects plant development (A) and rescues the high levels of infectivity of CMV‐Δ2b (B). Two independent transgenic 2b and 2blm lines are shown and were mechanically inoculated with CMV‐Δ2b at a concentration of 20 μg/mL. Total RNAs were extracted at 3 weeks after inoculation and used for Northern blotting. The extracted proteins were used in Western blots to assess the accumulation of CMV CP and transgenic 2b proteins. Methylene blue‐stained rRNA and Coomassie brilliant blue (CBB)‐stained protein were used as loading controls for RNA and protein, respectively. NT and V indicate non‐transgenic plants and empty vector transgenic plants, respectively. CMV, Cucumber mosaic virus; CP, coat protein.