Table 1.
Primers used in this study
| DNA or RNA Oligos | Sequences | Putative function |
|---|---|---|
| siGFP‐1 RNA | 5′GUCACUACUAUGGGUUAUGAG3′ | 21‐bp duplex siRNAs for EMSA |
| siGFP‐2 RNA | 5′CAUAACCCAUAGUAGUGACUG3′ | |
| 55‐nt sense RNA | 5′GAUGCAUUGUGCACUACGACUACUGCUCACUCGACGUAUAUAUCAGGCGACACAG3′ | Long dsRNA for EMSA |
| 55‐nt antisense RNA | 5′CUGUGUCGCCUGAUAUAUACGUCGAGUGAGCAGUAGUCGUAGUGCACAAUGCAUC3′ | |
| 2b‐F1 | 5′CAAGCTTATGGAATTGAACGTAGGTGC3′ | pGD‐2b/2blm/2bl/2bm |
| 2b‐R1 | 5′CGGATCCTCATTTGTCGTCATCGTCTTTG3′ | |
| 2b‐F2 | 5′TCCCCCGGGATGGAATTGAACGTAGGTGC3′ | 35S‐6myc‐Fny2blm |
| 2b‐R2 | 5′CGAGCTCTCAGGCATAGTCTGGGACGTC3′ | |
| 2bN61‐F1 | 5′CGCGGATCCATGGAATTGAACGTAGG3′ | pGEX‐KG‐Fny2b N61/2blmN61 |
| 2bN61‐R1 | 5′CCCAAGCTTTCAATCCACTTGATAGAACG3′ | |
| 2bN61‐F2 | 5′ CATGCCATGGATGGAATTGAACGTAGGTG 3′ | pET28a‐ Fny2b N61/2blmN61 |
| 2bN61‐R2 | 5′ CCGCTCGAGGAAAGCACCTTCCG 3′ | |
| GFP‐F | 5′TAATACGACTCACTATAGACGGAAACATCCTCGGCCAC3′ | GFP probe for Northern blot |
| GFP‐R | 5′TTATTTGTATAGTTCATCCATGCCATG3′ | |
| 2blm‐F | 5′CGTGCGGTGGAGGCGAAGAAGCAGAG3′ | Obtain CMV‐2blm |
| 2blm‐R | 5′AGCCGCTTGGAGTTCGACGTTTGTCATTG3′ | |
| 2bl‐F | 5′CGTATGGTGGAGGCGAAGAAGCAGAG3′ | With 2blm‐R to obtain CMV‐2bl |
| 2bm‐R | 5′AGCCAGTTGGAGTTCGACGTTTG3′ | With 2blm‐F to obtain CMV‐2bm |
CMV, Cucumber mosaic virus; dsRNA, double‐stranded RNA; EMSA, electrophoretic mobility shift assay; GFP, green fluorescent protein; nt, nucleotide; siRNA, small interfering RNA.