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. 2015 Jul 30;17(3):412–426. doi: 10.1111/mpp.12290

Figure 5.

figure

LTP3 mediates abscisic acid (ABA) signalling via AAO3 in seed germination. (A) ABA dose–response analysis of germination in Col‐0, ltp3‐1 and LTP3‐OX lines. Seeds were plated on Murashige and Skoog (MS) medium containing various amounts of ABA, and the germinative greening rate was scored 5 days after stratification by counting the seedlings with greening cotyledons. Data are means of three independent experiments ± standard deviation (SD) (each with at least 100 seeds for each line). (B) LTP3‐OX hypersensitivity to ABA on MS medium containing 0.5 μm ABA. Photographs were taken 7 days after stratification. (C) aao3 reduced LTP3‐OX69 hypersensitivity to ABA. Seeds were germinated and grown on MS medium containing 0.5 μm ABA for 7 days. Data are means ± SD from three biological replicates. Different letters above SD indicate significant differences, as determined by Fisher's protected least significant difference (LSD) test (P < 0.05). (D) Photographs were taken 9 days after stratification.