Skip to main content
. 2014 Jul 9;16(1):83–91. doi: 10.1111/mpp.12161

Figure 3.

figure

Performance of monopartite gene switch in Phytophthora infestans. (A) Linearized maps of plasmids expressing the VP16‐DBD‐EcR chimera (top) and β‐glucuronidase (GUS) reporter (bottom). Each also bears a neomycin phosphotransferase II (nptII) gene for selection of transformants (not shown). Transcription units employ the constitutive Ham34 promoter from Bremia lactucae (5'HAM), the minimal NifS promoter from P. infestans (PNIF) and the Ham34 transcriptional terminator (3'HAM). (B) Histochemical staining for GUS in hyphal plugs incubated for 24 h in rye–sucrose broth containing 0, 0.01 or 1 mm methoxyfenozide. Strains D1–D31 were obtained by co‐transformation of the two plasmids shown in (A); NC1 and NC2 are negative control transformants obtained using the GUS plasmid alone; CC is a positive control that expresses GUS from the Ham34 promoter. (C) Specific activity of GUS in transformants grown for 24 h in rye–sucrose broth containing 10 μm methoxyfenozide or the dimethylsulphoxide (DMSO) solvent alone, expressed as relative fluorescence units (RFU) per microgram. Values are from two biological replicates.