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. 2014 Jul 9;16(1):83–91. doi: 10.1111/mpp.12161

Figure 5.

figure

Tests of two‐hybrid switch expressed from a single plasmid. (A) Linearized map of plasmid encoding the GAL4 DBD‐EcR chimera, VP16‐RXR fusion and β‐glucuronidase (GUS) reporter. Not shown is neomycin phosphotransferase II (nptII). (B) Histochemical staining of GUS in transformants (S4–S111) containing the two‐hybrid plasmid and a strain expressing GUS from the Ham34 promoter (CC). Cultures were grown as in Fig. 3 with 0, 0.01 or 1 mm inducer. (C) Specific activity of GUS in transformants grown in broth containing 10 μm methoxyfenozide or the dimethylsulphoxide (DMSO) solvent alone, expressed as relative fluorescence units (RFU) per microgram of protein. Numbers above the bars represent the fold induction by methoxyfenozide.