Figure 7.

Induction of two‐hybrid reporter in two‐hybrid transformants S64 and S47 in hyphae and germinating sporangia. (A) β‐Glucuronidase (GUS) specific activity after treatment with 10 and 30 μm of inducer, or the dimethylsulphoxide (DMSO) solvent control. Left: the inducer was added to hyphae submerged in rye–sucrose broth, and tissue was analysed after 24 h. Centre: surface‐grown hyphae from agar cultures were transferred to medium containing inducer and assayed after 24 h. Right: sporangia were placed in 0.25 mm CaCl₂ containing inducer, incubated for 90 min at 12 °C and assayed. Specific activity is expressed as picomoles of substrate cleaved per second per milligram of protein. (B) Histochemical staining of hyphae induced on agar medium, or chilled sporangia.