Figure 8.

Performance of two‐hybrid system in tomato tissue infected with zoospores of Phytophthora infestans transformants, using the histochemical staining assay. (A) Leaf discs floated on water containing 10 μm methoxyfenozide (Meth) or the dimethylsulphoxide (DMSO) solvent control at 4 days post‐infection (dpi). In the S64 panel, P. infestans hyphae are starting to emerge from stomata (S) onto the leaf surface. In the S47 panel, P. infestans is shown at a slightly earlier stage of the disease cycle, in the apoplastic space. (B) Leaflets at 5 dpi from infected whole tomato plants sprayed with 10 μm methoxyfenozide or the control. (C) Same as (B) but at 3 dpi, showing two representative leaflets. (D) Leaflets from whole plants induced by adding methoxyfenozide to the soil. Strains were all‐in‐one transformants (S64, S74) and the constitutive β‐glucuronidase (GUS) strain (CC). Size bars in (A–C) are 40 μm.