Figure 2.
P0 proteins elicit hypersensitive response (HR)‐like cell death in Nicotiana glutinosa. (A) Transient over‐expression of the P0T u protein elicited cell death in Turnip yellows virus (TuYV)‐recognizing N. glutinosa line TW59. Leaves were infiltrated with Agrobacterium carrying binary vectors for transient expression of P0T u, the Tobacco mosaic virus (TMV) helicase (P50) recognized by the N gene and Rx plus its elicitor, the Potato virus X coat protein (PVX CP), or empty vector pBIN61. Leaves were photographed 5 days after infiltration. (B) To determine whether the P0 proteins from other viruses could also elicit HR in N. glutinosa, leaves from N. benthamiana and N. glutinosa accessions TW59 and TW61 were co‐infiltrated with the P0 proteins and green fluorescent protein (GFP). Agroinfiltrated leaves were photographed under white light to detect cell death and under UV light to show GFP accumulation enabled by P0 viral suppressor of RNA silencing (VSR) activity at 6 days post‐infiltration (dpi). The pBIN61 empty vector was co‐infiltrated with pBIN‐GFP as a negative control for HR induction and silencing suppression.