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. 2014 Oct 22;16(5):435–448. doi: 10.1111/mpp.12201

Figure 6.

figure

ARGONAUTE1 (AGO1)‐targeting viral suppressors of RNA silencing (VSRs) from other viruses failed to induce hypersensitive response (HR)‐like cell death in Nicotiana glutinosa. Plants were co‐infiltrated with green fluorescent protein (GFP) and VSR genes cloned into pBIN61 to test whether HR induction in N. glutinosa could be elicited generally by VSRs targeting AGO1. P0 proteins and Tobacco mosaic virus (TMV) P50 were included as positive controls for HR‐like cell death on TW59 (all P0 proteins) and TW61 (P0PL and P50). Potato virus X (PVX) P25 has been shown previously to target AGO1 for degradation (Chiu et al., 2010), whereas Cucumber mosaic virus (CMV) 2b and Turnip crinkle virus (TCV) P38 inhibit AGO1 activity through binding (Azevedo et al., 2010; Zhang et al., 2006). Leaves were photographed at 6 days post‐infiltration (dpi) under white (left) and UV (right) light to visualize HR‐like cell death and VSR activity through GFP accumulation.