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. 2015 Oct 20;17(4):577–587. doi: 10.1111/mpp.12303

Figure 1.

figure

Expression profiles of P hytophthora cactorum  PcF/small cysteine‐rich (SCR) effector‐encoding genes. (a) The gene expression level was determined by calculating the number of reads for each gene and then normalizing this to the reads per kilobase of exon model per million mapped reads (RPKM). The RPKM values of scr96, scr99, scr121 and PcF were calculated in this study using the RNA‐Seq data (GenBank SRA accession no. SRP059996). (b) The expression of scr96, scr99 and scr121 was profiled using reverse transcription‐polymerase chain reaction (RT‐PCR) throughout the P . cactorum life cycle, including mycelia (MY), sporangium (SP), zoospore (ZO) and germinating cyst (GC), and during infection stages, including 1.5, 3, 6, 12, 24, 48 and 96 h post‐inoculation (hpi) of N icotiana benthamiana. W, water used as PCR template; G, P . cactorum genomic DNA; PcU bc, internal control PcU bc gene of P . cactorum. Three independent biological experiments were performed and yielded similar results.