Figure 2.

Transient assays of P hytophthora cactorum  PcF/small cysteine‐rich (SCR) effectors in Solanaceae plants. The leaves of three species of Solanaceae plants, N icotiana benthamiana (a–d), tomato (e–h) and N . tabacum (i), were infiltrated with A grobacterium tumefaciens (strain GV3101) cells to express the effectors. The expression of scr96 triggered plant cell death (PCD) in both N . benthamiana (a) and tomato (e), whereas scr99 (f) and scr121 (g) induced PCD only in tomato. SCR99 (b) and SCR121 (c) did not induce PCD in N . benthamiana. Together with SCR96, these three cannot trigger PCD in N . tabacum (i). The P . cactorum protein PcF cannot cause PCD in any type of plant (d, h, i). The P . infestans elicitin INF1 served as a positive control in both N . benthamiana and N . tabacum (a–d, i), but not in tomato (h). The marker gene gfp and 10 mm MgCl₂ buffer were used as negative controls in all tested plants. The broken circles indicate the agroinfiltration areas without PCD, whereas irregular shaped areas are tissues in which PCD occurs. Photographs were taken 6 days (a–d) or 4 days (e–i) after infiltration.