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. 2015 Nov 11;17(5):741–754. doi: 10.1111/mpp.12326

Figure 2.

figure

Quantification of fungal biomass in planta by quantitative polymerase chain reaction (qPCR). Fungal DNA was quantified relative to plant DNA and relative to S porisorium reilianum f. sp. reilianum (SRS) DNA (A) or to S . reilianum f. sp. zeae (SRZ) DNA (B). Samples from the inoculation site, ligule, leaf sheath and stems were collected at 9 days after inoculation (dai) from sorghum and maize. (A) In sorghum, a greater amount of DNA from SRS than from SRZ was detected in ligules, leaf sheaths and stems. (B) In maize, SRZ was dominant in ligules, leaf sheaths and stems. The presence of SRS was observed in maize stems, whereas SRZ could not be detected in sorghum stems. The experiment was performed in three biological replicates of 10 plants each that were inoculated with SRS, SRZ or water. Error bars represent the standard error of the mean (SEM). Different letters above the bars indicate a significant difference (t‐test with P ≤ 0.05) within one tissue. *P value comparing the data of SRS and SRZ at the injection hole is slightly above 0.05.