Figure 4.

H₂O₂ accumulation at S porisorium reilianum infection sites in maize and sorghum. Samples were collected at 1 day after inoculation (dai) and were stained with 3,3′‐diaminobenzidine (DAB) and calcofluor. (A) Examples of appressoria showing H₂O₂ deposition (left, white arrow) and without H₂O₂ (right). (B, C) Percentage of appressoria with H₂O₂ deposition for sorghum (B) and maize (C). Three biological replicates containing three to four plants each were analysed. Data given are the mean values and standard deviations. The total number of counted appressoria is indicated above the bars. Different letters above the columns indicate significant differences (t‐test with P ≤ 0.05).